Hsyue-Jen Hsieh Professor

謝學真 教授

phone:(02)2363-3097 or (02)3366-3994

email: hjhsieh



Lab Info

Biomedical Engineering Laboratory



Education Background

B.S.Ch.E. National Taiwan University, 1983
M.S.Ch.E. National Taiwan University, 1985
Ph.D.Ch.E.Pennsylvania State University, 1992

Research Topic

Animal Cell Culture Engineering
The use of animal cell cultures to produce high value products like growth factors or blood clot dissolving agents has become a rapidly expanding technology. We are interested in studying the attachment and proliferation of anchorage-dependent animal cells and the effects of various influencing factors such as the materials of culture substrata, extracellular matrix molecules, culture medium composition, and shear force.


Modulation of Endothelial Cell Function by Shear Flow
Vascular endothelial cells line the blood vessel wall and are directly subjected to hemodynamic shear force. Many studies indicate that shear force experienced by the endothelial cells is involved in the regulation of vascular physiology. Moreover, these cells also play an important role in vascular diseases such as atherosclerosis. By using a parallel-plate flow chamber, we are currently investigating the effects of shear flow on the intracellular reactive oxygen species, protein kinases, gene expression, and other events.


Enzyme Technology
Enzymes are biocatalysts that find many applications in industry, clinical analyses, etc. Our main research interests in enzyme technology concentrate on the applications of immobilized enzymes and the denaturation and renaturation processes of enzymes and other proteins. We have developed a new technique using chitosan as a solid carrier for covalent coupling of invertase via carbohydrate moiety that improves the activity and thermal stability of immobilized invertase.  

Recent Research Topic

1.鹼性多醣/酸性多醣複合生醫材料之製備、分析及應用 H.J. Hsieh, sponsored by 97-2221-E-002-023-MY2 NT$ 1,084,000, 8/1/2009-7/31/2010


The Representative Publication Recent 5-years Publication

1. C.Y. Hsieh, H.Y. Hsiao, W.Y. Wu, C.A. Liu, Y.C. Tsai, Y.J. Chao, D.L. Wang, H.J. Hsieh, “Regulation of shear-induced nuclear translocation of the Nrf2 transcription factor in endothelial cells,” Journal of Biomedical Science 16, 12 (2009). (SCI)

2. N.Y. Yuan, Y.A. Lin, M.H. Ho, D.M. Wang, J.Y. Lia, H.J. Hsieh, “Effects of the cooling mode on the structure and strength of porous scaffolds made of chitosan, alginate, and carboxymethyl cellulose by the freeze-gelation method,” Carbohydrate Polymers 78, 349-356 (2009). (SCI) (合著)

3. P.H. Chen, T.Y. Kuo, D.M. Wang, J.Y. Lai, H.J. Hsieh, Use of Chitosan as a Material Stabilizer for Acidic Polysaccharides, Materials Science Forum 638-642, 570-575 (2010) (EI) (合著)

4. S.W. Hsiao, D.V.M. Thien, M.H. Ho, H.J. Hsieh, C.H. Li, C.H. Hung, H.H. Li, “Interactions between chitosan and cells measured by AFM,” Biomedical Materials 5, 054117 (8pp) (2010). (SCI, EI)

5. P.H. Chen, T.Y. Kuo, J.Y. Kuo, Y.P. Tseng, D.M. Wang, J.Y. Lai, H.J. Hsieh, “Novel chitosan–pectin composite membranes with enhanced strength, hydrophilicity and controllable disintegration,” Carbohydrate Polymers 82, 1236-1242 (2010). (SCI, EI)

6. H.K. Wang, C.H. Tsai, K.H. Chen, C.T. Tang, J.S. Leou, P.C. Li, Y.L. Tang, H.J. Hsieh, H.C. Wu, C.M. Cheng, “Cellulose-based diagnostic devices for diagnosing serotype-2 dengue fever in human serum,” Advanced Healthcare Materials (in press). (SCI)

7. R.Y. Tsai, S.C. Hung, J.Y. Lai, D.M. Wang, H.J. Hsieh, “Electrospun chitosan-gelatin-polyvinyl alcohol hybrid nanofibrous mats: production and characterization,” Journal of the Taiwan Institute of Chemical Engineers (in press). (SCI, EI)

8. R.Y. Tsai, P.W. Chen, T.Y. Kuo, C.M. Lin, D.M. Wang, T.Y. Hsien, H.J. Hsieh, “Chitosan/pectin/gum Arabic polyelectrolyte complex: Process-dependent appearance, microstructure analysis and its application,” Carbohydrate Polymers 101, 752-759 (2014). (SCI, EI)

9. Hsieh HJ, Liu CA, Huang B, Tseng AHH, Wang DL. “Shear-induced endothelial mechanotransduction: the interplay between reactive oxygen species (ROS) and nitric oxide (NO) and the pathophysiological implications,” Journal of Biomedical Science 21, 3 (2014). (SCI)