Professor


 

Ling Chao Associate Professor

趙 玲 副教授

phone:(02)33663023

email: lingchao

office:116A

 

Lab Info

Biomimetic Membrane Interfacial Phenomena and Engineering Lab

生技505,506,514

(02)33669936

Education Background

B.S. Ch.E. National Taiwan University, 2003

Ph.D. Ch.E. Massachusetts Institute of Technology, 2009

Post doc. Cornell University, 2009-2011


Research Topic

Phase Transformation Phenomena of Bio-membranes

Cell membranes are composed of various types of lipids and proteins. Phase separation, or called lipid membrane heterogeneity, exists in this multi-component system. The phase dynamics has been suggested to influence many cellular processes. Incorporating microfluidic and microfabriaction techniques, we can have control over lipid membrane’s pre-determined constituents, the spatial organization of membranes and proteins, and when different biomolecules interact with each other. The spatio-temporal control, which is difficult to achieve in conventional methods, allows us to obtain fundamental understanding of bio-membrane related cellular processes and to discover new biophysical phenomena. Current research directions include     exploring how phospholipases influence membrane phase behaviors and applying the newly discovered phase phenomena to liposome based drug delivery methods.


 

Cell Membrane Mimic Biochips

Our general research interest is to develop cell membrane mimic platforms to understand the underlying mechanisms of membrane related cellular processes for novel therapeutic applications. Cell membranes are the gateway for cells to send and receive signals and for pathogen infection. Membrane proteins constitute a significant portion of the human genome and are primary targets for drug candidates. However, membrane proteins are difficult to study, since their structures, functions, and activities are highly sensitive to their lipid membrane environments. Separating them from lipid membranes for characterization usually requires the use of detergents or other harsh methods, usually destroying protein function and structure. In addition, evidence has suggested that many membrane proteins can only function properly with lipid membranes. Cell membrane mimic platforms allow these proteins to be studied in their native environments. Current research topics include developing composite platforms and high-throughput platforms to examine how the membrane catalyzes structure changes of peripheral proteins and to screen rapidly how environmental conditions influence these proteins.



Recent Research Topic

Current research programs include:

Studying phase transformation phenomena in bio-membranes

Exploring physical pathway for potential therapeutic applications of phospholipase related disease.

Liposome based drug delivery application


Bio-interface engineering for studying peripheral protein-lipid membrane interactions

Use microfluidic and microfabrication techniques to develop composite membranes to study how the membrane catalyzes membrane protein structure changes

High throughput screening of environmental conditions in microfluidic devices


Honor


 
The Representative Publication Recent 5-years Publication

1. Chung-Ta Han and Ling Chao*, “Using a Patterned Grating Structure to Create Lipid Bilayer Platforms Insensitive to Air Bubbles”, Lab on a chip, 2015, 15(1), 86-93 (IF: 6.0) (SCI) (featured on the front cover page of issue 1, 2015)
2. Po-Yu Peng, Po-Chieh Chiang, and Ling Chao*, ”Mobile Lipid Bilayers on Gold Surfaces through Structure-Induced Lipid Vesicle Rupture”, Langmuir, 2015, 31(13), 3904-3911 (IF: 4.3) (SCI)
3. Cheng-yu Kuan, Po-Yu Peng, and Ling Chao*, “運用延伸DLVO理論來預測能將支撐式脂質雙層膜形成於非傳統基材之實驗條件”, 化工,2015, 62卷1期,103 - 117 
4. Shu-Kai Hu, Ya-Ming Chen and Ling Chao*, “Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species”, Biomicrofluidics, 2014, 8, 052005(1-11) (IF: 3.77) (SCI)
5. Po-Yu Peng, Po-Chieh Chiang, and Ling Chao*, “Controllable Occurrence of Free-Standing Lipid Membranes on Nanograting Structured Supports”, ACS Appl. Mater. Interfaces, 2014, 6 (15), 12261-12269 (IF: 5.9) (SCI)
6. Chung-Ta Han and Ling Chao*, “Creating Air-Stable Supported Lipid Bilayers by Physical Confinement Induced by Phospholipase A2”, ACS Appl. Mater. Interfaces, 2014, 6 (9), 6378-6383 (IF: 5.9) (SCI)
7. Yu-Ming Tu, Ling Chao*, “仿細胞膜平台的發展及應用”, 化工,2014, 61卷1期, 59-72  
8. Charng-yu Lin, Ling Chao*, “Tunable Nucleation Time of Functional Sphingomyelinase-Lipid Features Studied by Membrane Array Statistic Tool”, Langmuir, 2013, 29 (42), 13008-13017 (IF: 4.38) (SCI)
9. Shu-Kai Hu, Ling Chao*, “Using Crosslinkable Diacetylene Phospholipids to Construct Two-dimensional Packed Beds in Supported Lipid Bilayer Separation Platforms”, Sci. Technol. Adv. Mater., 2013, 14, 044408 p1-p8 (IF:3.75) (SCI) 
10. Ling Chao, Mark Richards, C.-Y. Hsia, and Susan Daniel, “Two-Dimensional Continuous Extraction in Multiphase Lipid Bilayers To Separate, Enrich, and Sort Membrane-Bound Species”, Analytical Chemistry, 2013, 85, 6696-6702 (IF: 5.7) (SCI)
11. Ling Chao, Susan Daniel, “Measuring the Partitioning Kinetics of Membrane Biomolecules Using Patterned Two-phase Coexistent Lipid Bilayers”, Journal of the American Chemical Society, 2011, 133 (39), 15635-15643 (IF:10.7) (SCI)
12. Ling Chao, Klavs F. Jensen*, T. Alan Hatton*, “Two-dimensional Solvent-Mediated Phase Transformation in Lipid Membranes Induced by Sphingomyelinase”, Langmuir, 2011, 27, 10050-10060 (IF:4.38) (SCI)
13. Ling Chao, Susan Daniel*, “Supported Lipid Bilayer Electrophoresis for Separation and Analytical Studies of Cell Membrane Biomolecules”, peer-reviewed book chapter in Interfaces and Interphases in Analytical Chemistry, Helburn and Vitha (Ed.), ACS Symposium Series, 2011, 99-121 
14. Ling Chao and Susan Daniel*,” Device for Sorting, Classifying, and Assaying Partition Behavior of Cell Membrane Biomolecules and Methods Based Thereon”, US 20130095512 A1, WO/2011/160071
15. Ling Chao, Alice P. Gast, T. Alan Hatton, Klavs F. Jensen*, “Sphingomyelinase-Induced Phase Transformations: Causing Morphology Switches and Multiple-Time-Domain Ceramide Generation in Model Raft Membranes”, Langmuir, 2010, 26, 344 (IF:4.38) (SCI)