Hwai-Shen Liu Professor

劉懷勝 教授


email: hsliu



Lab Info

Bioprocessing Engineering Laboratory



Education Background

S.Ch.E. National Taiwan University, 1982
M.S.Ch.E. University of Tennessee, 1987
Ph.D.Ch.E.University of Tennessee, 1990

Research Topic

Bioprocess Engineering

Our research focuses on the bioprocess engineering which includes both fermentation and separation processes. In the fermentation technology, efforts have been devoted to renewable resources utilization, waste oil biodegradation, periodical operation strategy and its associated dynamic behavior, in-situ inhibitor removal, insect ell/baculovrius expression system for recombinant protein (interleukin-5), serum-free media development, fermentation additive to enhance oxygen transfer and productivity. The applications of isoamylase were also investigated, which covers immobilization techniques and enhanced reaction under supercritical carbon dioxide. For downstream process, proteins/enzymes separation by adsorption, foam fractionation and chromatographic method as well as stability enhancement and refolding have been investigated.


Higee System

Process intensification by Higee system (rotating packed bed) could enhance mass transfer greatly; therefore, reduce the equipment size dramatically. Attractive applications in absorption, stripping, adsorption, are being experimentally demonstrated in our research results. Meanwhile, fundamental studies of design criteria are currently investigated.

Recent Research Topic

2.綠色化學程序」尖端技術科專計劃 第二期(3/3)


The Representative Publication Recent 5-years Publication

1. W. N. Chang, C. W. Liu, and H. S. Liu, “Hydrophobic Cell Surface and Bioflocculation Behavior of Rhodococcus erythropolis Sea Water” Process Biochemistry 44, 955-962 (2009) (SCI, EI)

2. C. Y. Tai, Y. H. Wang, Y. W. Kuo, M. H. Chang, H. S. Liu, “Synthesis of silver particles below 10nm using spinning disk reactor” Chemical Engineering Science 64, 3112 – 3119 (2009) (SCI, EI)

3. C. W Liu, W. N. Chang, and H. S. Liu, “Bioremediation of n-alkanes and the Formation of Biofloccules by Rhodococcus erythropolis NTU-1 under Various Saline Conditions and Sea Water”, Biochem. Eng. J. 45, 69–75 (2009) (SCI, EI)

4. S. S. S. Wang, H. S. Chao, H. L. Liu, and H. S. Liu, Stability of Hen Egg White Lysozyme during Denaturation is enhanced via Pretreatment in Supercritical Carbon Dioxide Journal of Bioscience and Bioengineering 107, 355-359 (2009) (SCI, EI) (合著)

5. E. J. Hsu, J. F. Hsu, S. L. Landuyt, H. S. Chao and H. S. Liu, “Semi-Continuous Propagation and Synchronous Differentiation of Hyper-Solventogenic Cells of Clostridium thermosaccharolyticum in a Xylan Medium at 56oC” J. Tw. Inst. Chem. Engrs. 40, 349-358 (2009) (SCI, EI)

6. C. Y. Chiang, Y. S. Chen, M. S. Liang, F. Y. Lin, C. Y. D. Tai and H. S. Liu, “Absorption of Ethanol into Water and Glycerol/Water Solution in a Rotating Packed Bed” J. Tw. Inst. Chem. Engrs. 40, 418-423 (2009)(SCI, EI)

7. S. S. S. Wang, J. T. Lai, M. S. Huang, C. Y. Tai, and H. S. Liu, Deactivation of isoamylase and beta-amylase in the agitated reactor under supercritical carbon dioxide Bioprocess Biosyst Eng, 33, 1007-1015 (2010) (SCI, EI) (合著)

8. Y. S. Chen, Y. H. Wang, H. S. Liu, K. Y. Hsu, and C. Y. Tai, “Micronization of p-Aminosalicylic Acid Particles Using High-Gravity Technique” Industrial & Engineering Chemistry Research 49, 8832-8840 (2010) (SCI, EI)

9. C. W. Liu, and H. S. Liu, “Rhodococcus erythropolis strain NTU-1 efficiently degrades and traps diesel and crude oil in batch and fed-batch bioreactors” Process Biochemistry 46, 202-209 (2011) (SCI, EI)

10. Y. C. Chen and H. S. Liu, “Considerations of chaperon solvent plug design in protein refolding by size-exclusion chromatography” Enzy. Mircrob. Tech. 49, 203-208 (2011) (SCI, EI)

11. M. H. Chang, H. S. Liu, and C. Y. Tai, “Preparation of Copper Oxide Nanoparticles and Its Application In Nanofluid” Powder Technology 207, 378–386 (2011) (SCI, EI)

12. H. S. Liu, Y. H. Wang, C. C. Li and C.Y. Tai, “Characterization of AgI nanoparticles synthesized in a spinning disk reactor” Chem. Eng. J. 183, 466-472 (2012) (SCI, EI)

13. C. W. Liu, M. S. Liang, Y. C. Chen, L. Sayavedra-Soto and H. S. Liu, “Biodegradation of n-alkanes at high concentration and correlation to the accumulation of H+ ions in Rhodococcus erythropolis NTU-1” Biochem. Eng. J. 63, 124-128 (2012) (SCI, EI)

14. K. N. Liu, C. M. Lai, Y. T. Lee, S. N. Wang, R. P. Y. Chen, J. S. Jan, H. S. Liu, S. S. S. Wang, “Curcumin's Pre-incubation Temperature Affects Its Inhibitory Potency toward Amyloid Fibrillation and Fibril-induced Cytotoxicity of Lysozyme” Biochimica et Biophysica Acta 1820,17741786 (2012) (SCI, EI) (合著)

15. C. Y. Chiang, Y. Y. Liu, Y. S. Chen, H. S. Liu, “Absorption of Hydrophobic Volatile Organic Compounds by a Rotating Packed Bed” Industrial & Engineering Chemistry Research 51 (27), 9441–9445 (2012) (SCI, EI)

16. Y. C. Chen, W. T. Lin, J. Wu and H. S. Liu, “Efficient lysozyme protein refolding at high final concentration and low dilution factor” Process Biochemistry, 47, 1883-1888 (2012) (SCI, EI)

17. H. S. Liu, K. A. Chen and C. Y. Tai, “Droplet stability and product quality in the Higee-assisted microemulsion process for preparing CaCO3 particles” Chemical Engineering Journal, 197, 101-109 (2012) (SCI, EI)

18. C. Y. Chiang, M. H. Chang, H. S. Liu, C. Y. Tai, S. Ehrman, “Process Intensification in the Production of Photocatalysts for Solar Hydrogen Generation” Industrial & Engineering Chemistry Research, 51, 5207-5215 (2012) (SCI, EI)

19. H. Kurniawan, Y. S. Ye, W. H. Kuo, J. T. Lai, M. J. Wang, H. S. Liu, “Improvement of biofouling resistance on bacterial cellulose membranes” Biochemical Engineering Journal, 78(15): 138-145 (2013) (SCI, EI)

20. J. W. Wu, K. N. Liu, S. C. How, W. A. Chen, C. M. Lai, H. S. Liu, C. J. Hu, S. S. S. Wang, “Carnosine’s Effect on Amyloid Fibril Formation and Induced Cytotoxicity of Lysozyme” PLoS ONE 8(12): e81982.doi:10.1371 /journal.pone.0081982 (2013)