研究主題

動物細胞培養工程
動物細胞能生產多種高附加價值之生物產品，例如：生長因子、溶血栓藥物等。本研究室探討動物細胞附著及生長模式，以及各種影響因素（如：培養基材、胞外間質、培養液成份、剪力作用等）之效應。

 

剪力對內皮細胞之調控
血管內皮細胞位於血管最內層，直接承受血流產生之剪力，並據以調節血管生理功能，內皮細胞也在動脈硬化等血管病變過程中扮演重要角色。本研究室使用剪流裝置探討剪力對內皮細胞功能之調控，其中包括胞內活性氧族群、蛋白激酶、基因表現等之變化。

 

酵素應用
酵素之應用範圍相當廣泛，包括了工業生產、臨床醫療等方向。本研究室以轉化酵素為模式，比較各種固定化方法及載體之影響。另外亦探討溶菌酵素復性過程，設法以透析等方式提高其復性效果，改良目前採用之稀釋復性程序。

最新研究

1. 鹼性多醣/酸性多醣複合生醫材料之製備、分析及應用 H.J. Hsieh, sponsored by 97-2221-E-002-023-MY2 NT$ 1,233,000, 8/1/2008-7/31/2009

榮譽獎項

1. 黃玲惠，陳柏仰，謝學真，「多孔狀膠原蛋白基質之製備方法」，中華民國發明專利第150746號，(2002/2-2020/10)。 2. 賴君義、何明樺、郭佩芸、謝學真、謝子陽、王大銘，「生物基材之形成方法」，中華民國發明專利第233943號，(2005/6-2023/12)。

 

代表著作 所有著作

1. M.H. Ho, P.Y. Kuo, H.J. Hsieh, T.Y. Hsien, L.T. Ho, and D.M. Wang, “Preparation of Porous Scaffolds by Using Freeze-Extraction and Freeze-Gelation Methods,” Biomaterials, 25, 129-138 (2004). (SCI, EI)

 

2. C.W. Ni, H.J. Hsieh, Y.J. Chao, and D.L. Wang, “Interleuk-6 induced JAK2/STAT3 Signaling Pathway in Endothelial Cells Is Suppressed by Hemodynamic Flow,” Am. J. Physiol. Cell Physiol., 287, C771-C780 (2004). (SCI)

 

3. M.H. Ho, D.M. Wang, H.J. Hsieh, H.C. Liu, T.Y. Hsien, J.Y. Lai, L.T. Hou, “Preparation and characterization of RGD-immobilized chitosan scaffolds,” Biomaterials, 26, 3197-3206 (2005). (SCI, EI)

 

4. C.Y. Hsieh, S.P. Tsai, D.M. Wang, Y.N. Chang, H.J. Hsieh, “Preparation of -PGA/chitosan composite tissue engineering matrices,” Biomaterials, 26, 5617-5623 (2005). (SCI, EI)

 

5. Y.C. Wang, R.H. Fu, H.J. Hsieh, H.T. Chao, S.H. Kao, “Polyglycolic acid/chitosan glue and apoptosis of endometriotic cells,” Fertil Steril, 84, 75-81 (2005). (SCI)

 

6. M.H. Ho, L.T. Hou, C.Y. Tu, H.J. Hsieh, J.Y. Lai, W.J. Chen, D.M. Wang, “Promotion of cell affinity of porous PLLA scaffolds by immobilization of RGD peptides via plasma treatment,” Macromol Biosci, 6, 90-98 (2006). (SCI, EI)(合著)

 

7. C.Y. Hsieh, H.J. Hsieh, H.C. Liu, D.M. Wang, L.T. Hou, “Fabrication and release behavior of a novel freeze-gelled chitosan/γ-PGA scaffold as a carrier for rhBMP-2,” Dent Mater, 22, 622-629 (2006). (SCI, EI)(合著)

 

8. M.H. Ho, D.M. Wang, L.T. Hou, H.J. Hsieh, J.Y. Lai, “Preparation of cell-specific membranes for bone regeneration by peptide grafting,” Desalination, 199, 144-146 (2006). (SCI, EI)(合著)

 

9. C.Y. Hsieh, S.P. Tsai, M.H. Ho, D.M. Wang, C.E. Liu, C.H. Hsieh, H.C. Tseng, H.J. Hsieh, “Analysis of freeze-gelation and cross-linking processes for preparing porous chitosan scaffolds,” Carbohydr Polym, 67, 124-132 (2007). (SCI, EI)

 

10. S.P. Tsai, C.Y. Hsieh, C.Y. Hsieh, Y.N. Chang, D.M. Wang, H.J. Hsieh, “Gamma-poly(glutamic acid)/chitosan composite scaffolds for tissue engineering applications,” Mater Sci Forum, 539-543, 567-572 (2007). (SCI, EI) (合著)

 

11. P.H. Chen, Y.H. Hwang, T.Y. Kuo, F.H. Liu, J.Y. Lai, H.J. Hsieh, “Improvement in the Properties of Chitosan Membranes Using Natural Organic Acid Solutions as Solvents for Chitosan Dissolution,” J Med Biol Eng, 27, 23-28 (2007). (EI)

 

12. M.H. Ho, J.J. Lee, S.C. Fan, D.M. Wang, L.T. Hou, H.J. Hsieh, J.Y. Lai, “Efficient Modification on PLLA by Ozone Treatment for Biomedical Applications,” Macromol Biosci, 7, 467-474 (2007). (SCI, EI) (合著)

 

13. J.L. Lin, R.C. Ruaan, H.J. Hsieh, “Refolding of partial and fully denatured lysozymes,” Biotechnol Lett, 29, 723-729 (2007). (SCI, EI)

 

14. Y.C. Tsai, H.J. Hsieh, F. Liao, C.W. Ni, Y.J Chao, C.Y. Hsieh, D.L. Wang, “Laminar flow attenuates interferon-induced inflammatory responses in endothelial cells,” Cardiovasc Res, 74, 497-505 (2007). (SCI)

 

15. S.P. Tsai, C.Y. Hsieh, C.Y. Hsieh, D.M. Wang, L.L.H. Huang, J.Y. Lai, H.J. Hsieh, “Preparation and cell compatibility evaluation of chitosan/collagen composite scaffolds with amino acids as cross-linking bridges,” J Appl Polym Sci, 105, 1774-1785 (2007). (SCI, EI) (合著)

 

16. P.Y. Chen, L.L.H. Huang, H.J. Hsieh, “Hyaluronan Preserves the Proliferation and Differentiation Potentials of Long-term Cultured Murine Adipose-Derived Stromal Cells,” Biochem Biophys Res Commun, 360, 1-6 (2007). (SCI)

 

17. 苑乃義、蔡睿逸、H.J. Hsieh謝學真，藥物控制釋放與幾丁聚醣微粒，化工，第54卷，第6期：112-119 (2007).

 

18. N.Y. Yuan, R.Y. Tsai, M.H. Ho, D.M. Wang, J.Y. Lai, H.J. Hsieh, “Fabrication and characterization of chondroitin sulfate-modified chitosan membranes for biomedical applications,” Desalination 234, 166-174 (2008). (SCI, EI) (合著)

 

19. P.H. Chen, T.Y. Kuo, Y.H. Hwang, F.H. Liu, M.H. Ho, D.M. Wang, J.Y. Lai, H.J. Hsieh, “Use of dicarboxylic acids to improve and diversify the material properties of porous chitosan membranes,” J Agric Food Chem 56, 9015-9021 (2008). (SCI) (合著)

 

 

 

Patents

 

1. 黃玲惠，陳柏仰，H.J. Hsieh謝學真，「多孔狀膠原蛋白基質之製備方法」，中華民國發明專利第150746號，(2002/2-2020/10)。

 

2. 賴君義、何明樺、郭佩芸、H.J. Hsieh謝學真、謝子陽、王大銘，「生物基材之形成方法」，中華民國發明專利第233943號，(2005/6-2023/12)