教授介紹


 

王勝仕 教授

Steven S.-S. Wang

電話(02) 3366-5870
傳真(02) 2362-3040

電子郵件 sswang

辦公室:117

 

研究室資料

生物分子工程研究室

308

(02) 3366-3057

學經歷

中央大學                 化學工程學士,1991

台灣大學                 化學工程碩士,1993

美國德州農工大學    化學工程博士,2002


研究主題

奈米技術與生物技術相結合主題

本實驗室著重於奈米技術與生物技術相結合之研究主題。主題之一在探討奈米粒子、奈米材料、及高分子材料與生物分子(如:細胞、蛋白質等)間之交互作用。其次,我們亦嘗試瞭解此些奈米粒子、奈米材料、及高分子材料對人體之影響及其毒害性。此外,我們也利用奈米粒子及高分子材料包覆藥物,建立藥物釋放系統,並探討其在疾病治療應用上之可行性。


 

聚集現象、蛋白質折疊/去折疊行為、及其應用

聚集為自然界中常見且重要的現象。蛋白質聚集往往與其折疊/去折疊行為有關。本研究室嘗試以不同之化學工程工具(如:動力學、熱力學、及輸送現象等)與生化方法來探討上述聚集現象與蛋白質折疊/去折疊之行為及相應分子機制。此外,亦將生物分子所形成之特殊聚集結構(如:類澱粉纖維)與材料科學、組織工程、及食品科學等領域相結合,並嘗試尋找其可能之應用領域(如:組織工程中之骨架(scaffold)、食品科學中之添加物(additives))


 

電腦模擬

我們亦利用電腦分子動態模擬、計算化學、生物資訊、理論模擬等方式,對上述相關主題進行探討,並將模擬結果與本研究室之實驗結果加以比較,期望對主宰此些重要程序之分子機制能有進一步之瞭解。

 

最新研究

1.Effect of Reduction-Oxidation Composition on the Fibrillogenesis of Amyloid Proteins. 氧化還原組成對於蛋白質類澱粉形成之影響 Sheng-Shih Wang, sponsored by National Science Council, NSC98-2221-E-002-063-MY3, 8/01/2009-7/31/2010.

 


榮譽獎項

1. National Taiwan University Distinguished Counseling Advisor Award, 2011 (台灣大學第五屆優良導師)

2. The Society of Chemical Engineering, Japan (SCEJ) Asia Research Award, Nominee, 2011

3. 化工系 99 學年度優良導師

4. 化工系 98 學年度優良導師

5. National Taiwan University Excellent Teaching Award, 2007 (工學院推薦 96 學年度教學優良教師)

6. National Taiwan University Excellent Teaching Award, 2006 (工學院推薦 95 學年度教學優良教師) 

7. Guest Editor, Journal of The Chinese Institute of Chemical Engineers (2005/6~2005/12)

8. 指導大專生參與專題研究計畫研究創作獎 ,陳雅婷 (2005)

 
代表著作 近五年著作


1. S. S.-S. Wang, K. N. Liu, C. H. Wu, and J. K. Lai, “Investigating the Influences of Redox Buffer Compositions on the Amyloid Fibrillogenesis of Hen Egg-White Lysozyme,” Biochimica et Biophysica Acta – Proteins and Proteomics, 1794(11), 1663–1672 (2009) (SCI).

2. S. S.-S. Wang, S. W. Chou, K. N. Liu, and C. H. Wu, “Effects of Glutathione on Amyloid Fibrillation of Hen Egg-White Lysozyme,” International Journal of Biological Macromolecules, 45(4), 321–329 (2009) (SCI).

3. S. S.-S. Wang, K. N. Liu, and W. H. Lee, “Effect of Curcumin on the Amyloid Fibrillogenesis of Hen Egg-White Lysozyme,” Biophysical Chemistry, 144(1-2), 78–87 (2009) (SCI).

4. U. L. Chen, and S. S.-S. Wang, Y. Y. Yang, R. Y. Yuan, R. M. Chen, and C. J. Hu, “The Epigenetic Effects of Amyloid-beta(1-40) on Global DNA and Neprilysin Genes in Murine Cerebral Endothelial Cells,” Biochemical and Biophysical Research Communications, 378(1), 57–61 (2009) (SCI).

5. M. S. Lin, X. B. Chen, S. S.-S. Wang, Y. Chang, and W. Y. Chen, “Dynamic Fluorescence Imaging Analysis to Investigate the Cholesterol Recruitment in Lipid Monolayer during the Interaction between β-amyloid (1–40) and Lipid Monolayers,” Colloids and Surfaces B: Biointerfaces, 74(1), 59–66 (2009) (SCI, EI).

6. S. S.-S. Wang, K. N. Liu, and B. W. Wang, “Effects of Dithiothreitol on the Amyloid Fibrillogenesis of Hen Egg-White Lysozyme,” European Biophysics Journal with Biophysics Letters, 39, 1229–1242 (2010) (SCI).

7. S. S.-S. Wang, Y. T. Hung, and Y. C. Lin, “Kinetic Studies of the Oxidation of Glutathione in Protein Refolding Buffer,” Bioprocess and Biosystems Engineering, 33, 277–286 (2010) (SCI, EI).

8. K. N. Liu, H. Y. Wang, C. Y. Chen, and S. S.-S. Wang, “L-Arginine Reduces Thioflavin T Fluorescence but not Fibrillation of Bovine Serum Albumin Amino Acids,” Amino Acids, 39, 821–829 (2010) (SCI).

9. S. S.-S. Wang, K. N. Liu, and T. C. Han, “Amyloid Fibrillation and Cytotoxicity of Insulin are Inhibited by the Amphiphilic Surfactants,” Biochimica et Biophysica Acta – Molecular Basis of Diseases, 1802, 519–530 (2010) (SCI).

10. P. T. Chen, T. Y. Liao, C. J. Hu, S. T. Wu, S. S.-S. Wang, and Rita P. Y. Chen, “A highly sensitive peptide substrate for detecting two A-degrading enzymes: neprilysin and insulin-degrading enzyme” Journal of Neuroscience Methods, 190, 57–62 (2010) (SCI).

11. S. S.-S. Wang, J. T. Lai , M. S. Huang, Clifford Y. Tai, H. S. Liu “Deactivation of isoamylase and β-amylase in the agitated reactor under supercritical carbon dioxide” Bioprocess and Biosystems Engineering, 33, 1007–1015 (2010) (SCI, EI). (合著)

12. J. K. Lai, T. H. Chuang, J. S. Jan, and S. S.-S. Wang, “Efficient and Stable Enzyme Immobilization in a Block Copolypeptide Vesicle-Templated Biomimetic Silica Support,” Colloids and Surfaces B: Biointerfaces, 80, 51–58 (2010) (SCI, EI).

13. Y. T. Hung, M. S. Lin, W. Y. Chen, and S. S.-S. Wang, “Investigating the Effects of Sodium Dodecyl Sulfate on the Aggregative Behavior of Hen Egg-White Lysozyme at Acidic pH,” Colloids and Surfaces B: Biointerfaces, 81, 141–151 (2010) (SCI, EI).

14. S. S.-S. Wang, M. S. Lin, W. Y. Chen, “Using Isothermal Titration Calorimetry to Real-time Monitor the Heat of Metabolism: A Case Study Using PC12 Cells and A(1-40),” Colloids and Surfaces B: Biointerfaces 83, 307–312 (2011) (SCI, EI).

15. W. S. Wen, M. C. Hsieh, and S. S.-S. Wang, “High-Level Expression and Purification of Human D-Crystallin in Escherichia coli,” Journal of the Taiwan Institute of Chemical Engineers 42(4), 547-555 (2011) (SCI, EI).

16. S. S.-S. Wang and W. S. Wen, “Examining the Influence of Ultraviolet C Irradiation on Recombinant Human γD-Crystallin,” Molecular Vision, 16, 2777–2790 (2010) (SCI).

17. S. S.-S. Wang, P. Wang, K. N. Liu, and W. S. Wen, “Fibril Formation of Bovine -Lactalbumin is Inhibited by Glutathione ,“ Food Biophysics 6, 138–151 (2011) (SCI).

18. S. S.-S. Wang, Y. T. Hung, K. C. Lin, G. Y. Chen, and W. S. Wen, “Exploring the Inhibitory Activity of Short-Chain Phospholipids against Amyloid Fibrillogenesis of Hen Egg-White Lysozyme,” Biochimica et Biophysica Acta–Molecular and Cell Biology of Lipids, 1811, 301–313 (2011) (SCI).

19. S. S.-S. Wang, J. K. Lai, Y. J. Lin, Y. J. Huang, and J. S. Jan, “Inhibition of Hen Egg-White lysozyme Amyloid Fibrillation by Copolypeptides,” Biopolymers, 97(2), 107–116 (2012) (SCI).

20. S. S.-S. Wang, M. C. Chang, H. C. Chang, M. H. Chang, and C. Y. Tai, “Growth Behavior of Aragonite under the Influence of Magnetic Field, Temperature, and Impurity,” Industrial & Engineering Chemistry Research (accepted) (SCI, EI). (合著)