研究主題

藥物基因傳遞
反轉錄病毒介導之基因傳遞 本研究室已驗證超音波駐波場可用來促進病毒轉導率。在駐波場內，懸浮細胞先被推引至壓力節點面，之後形成細胞群集並用以捕捉流轉在節點面間的病毒。藉此機制可提昇基因之傳遞率。為更進一步增加病毒轉導率，超音波設備的改良與操作參數的適化乃目前之研究方向。 非病毒型載體之研發 不同於廣在文獻上報導過的非病毒型載體，本研究室應用負離子型多醣體來研製攜帶藥物基因的奈米載體。此乃鑒於，〈一〉血漿蛋白質在攜帶藥物基因之奈米顆粒上的調理吸附作用將可能被帶負電之多醣體減低，因而使藥物基因載體較為穩定；〈二〉被細胞內噬的奈米顆粒會在內膜體中溶蝕；如此，因滲透壓差引起之內膜體漲大將可能加速藥物基因載體的逃逸率。攜帶藥物基因的多醣體奈米顆粒將經由水在油相之微乳化程序製成。多種設計的?數對奈米顆粒尺寸的影響將會做系統化的調查。完整特性化含藥物基因的多醣體奈米顆粒將會用來檢驗藥物基因傳遞之效率。

最新研究

1. Neovasculature-Targeted Apoptogenic RNAi Vectors (NSC 96-2628-E-002-013-MY3; 96/08/01~99/07/31)

2. Virus Incorporated with Quantum Dots as Nanobioprobes (National Health Research Institutes; 98/01/01~100/12/31)

 

榮譽獎項

1. Scientific Advisory Committee, Los Angeles Tissue Engineering Initiative (2004 – 2006)

2. Editorial Advisory Board, Open Biotechnology Journal (2007 – present)

代表著作 所有著作

1. You, J-O and Peng, C-A, Calcium-alginate nanoparticles formed by reverse microemulsion as gene carriers, Macromolecular Symposia 219: 147-153, 2004. (SCI, EI)

 

2. You, J-O and Peng, C-A, Phagocytosis-mediated retroviral transduction: Co-internalization of deactivated retrovirus and calcium-alginate microsphere by macrophage, Journal of Gene Medicine 7: 398-406, 2005. (SCI)

 

3. Lee, Y-H and Peng, C-A, Enhanced retroviral gene delivery in ultrasonic standing wave fields, Gene Therapy 12: 625-633, 2005. (SCI)

 

4. Lee, Y-H, You, J-O, and Peng, C-A, Retroviral transduction of adherent cells in resonant acoustic fields, Biotech Progress 21: 372-376, 2005. (SCI, EI)

 

5. Kwon, YJ and Peng, C-A, High-yield retroviral production using temperature modulated two-stage operation, Biotech and Bioeng 90: 365-372, 2005. (SCI, EI)

 

6. Lee, Y-H, Lai, C-Y, Li, P-C, Peng, C-A, Ultrasound-mediated perfluorocarbon microbubbles bursting for gene transfection, J. Medical Biol Eng 25: 153-158, 2005. (EI)

 

7. Peng, C-A, Retroviral gene delivery from engineering perspective, J. Chinese Inst Chem Eng 36:577-583, 2005. (SCI, EI)

 

8. You, J-O, Liu, Y-S, Liu, Y-C, Joo, K-I, Peng, C-A, Incorporation of quantum dots on virus in polycationic solution, Inter. J. Nanomedicine 1: 59-64, 2006.

 

9. Don, T-M, King, C-F, Chiu, W-Y, Peng, C-A, Preparation and characterization of chitosan-g-poly(vinyl alcohol)/poly(vinyl alcohol) blends used for the evaluation of blood-contacting compatibility, Carbohydrate Polymers 63: 331-339, 2006. (SCI, EI)

 

10. Peng, C-A, Da, J, Hsu, Y-C, Hogen-Esch, TE, Polymeric surfactant mediated perfluorocarbon emulsions, J. Dispersion Sci Tech 27: 377-387, 2006. (SCI, EI)

 

11. You, J-O, Liu Y-C, Peng, C-A, Efficient gene transfer using chitosan/alginate core-shell nanoparticles, Inter. J. Nanomedicine, 2: 173-180, 2006.

 

12. Lee, Y-H, Peng, C-A, Nonviral gene transfer of suspension cells in ultrasound standing wave fields, Ultrasound Med Biol 33: 734-742, 2007. (SCI)

 

13. Yacobi, NR, Phuleria, HC, Demaio, L, Liang, CH, Peng, C-A, Sioutas, C, Borok, Z, Kim, K-J, Crandall, ED, Nanoparticle effects on rat alveolar epithelial cell monolayer barrier properties, Toxicology in Vitro 21: 1373-1381, 2007. (SCI)

 

14. Peng, C-A, Huang, F, Formation of perfluorocarbon microemulsion using nonionic perfluoroalkylated polyethylene glycol surfactant, J. Dispersion Sci Tech 29: 46-51, 2008. (SCI, EI)

 

15. Kwon, YJ, Peng, C-A, Differential interaction of retroviral vector with target cell: quantitative effect of cellular receptor, soluble proteoglycan, and cell type on gene delivery efficiency, Tissue Eng Part A 14: 1497-1506, 2008. (SCI)

 

16.  Wang, C-H, Hsu, Y-S, Peng, C-A, Quantum dots encapsulated with amphiphilic alginate as bioprobe for fast screening anti-dengue virus agents, Biosens Bioelectron 24: 1018-1025, 2008. (SCI, EI)

 

17.  Peng, C-A, Analysis of gene transfer rate with immobilized retroviral vectors, Ann NY Acad Sci, in press.

 

 

 

Patents

 

1. Peng, C-A and Hsu, Y-C, Anti-Phagocytic Surfactants for Perfluorocarbon Emulsions, Taiwanese patent No. 89114488 (2000).

 

2. Peng, C-A and Hsu, Y-C, Perfluorocarbon-Soluble Surfactants (submitted to Office of Technology Licensing, USC file # 3321). It has been filed as “perfluorocarbon-soluble compounds” to the USPTO (Application #: 20060002881).

 

3. Peng, C-A, Microalgal Mass Culture Under High Carbon Dioxide Concentration and Light Intensity. It has been filed to the Taiwan Patent Office.

 

4. Peng, C-A, Quantum Dots-Tagged Viruses for Anti-Viral Drug Screening. It has been filed to the Taiwan Patent Office.